Hairpin RNAs and Retrotransposon LTRs Effect RNAi and Chromatin-Based Gene Silencing | Zendy

Véra Schramke | Zendy; Robin C. Allshire | Zendy

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Hairpin RNAs and Retrotransposon LTRs Effect RNAi and Chromatin-Based Gene Silencing

Author(s) -

Véra Schramke,

Robin C. Allshire

Publication year - 2003

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1086870

Subject(s) - biology , rna interference , retrotransposon , genetics , chromatin , small hairpin rna , rna silencing , rna , gene , rna induced silencing complex , gene silencing , microbiology and biotechnology , transposable element , genome

The expression of short hairpin RNAs in several organisms silences gene expression by targeted mRNA degradation. This RNA interference (RNAi) pathway can also affect the genome, as DNA methylation arises at loci homologous to the target RNA in plants. We demonstrate in fission yeast that expression of a synthetic hairpin RNA is sufficient to silence the homologous locus in trans and causes the assembly of a patch of silent Swi6 chromatin with cohesin. This requires components of the RNAi machinery and Clr4 histone methyltransferase for small interfering RNA generation. A similar process represses several meiotic genes through nearby retrotransposon long terminal repeats (LTRs). These analyses directly implicate interspersed LTRs in regulating gene expression during cellular differentiation.

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