Correlation Between Histone Lysine Methylation and Developmental Changes at the Chicken β-Globin Locus | Zendy

Michael D. Litt | Zendy; Melanie A. Simpson | Zendy; Miklós Gaszner | Zendy; C. David Allis | Zendy; Gary Felsenfeld | Zendy

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Correlation Between Histone Lysine Methylation and Developmental Changes at the Chicken β-Globin Locus

Author(s) -

Michael D. Litt,

Melanie A. Simpson,

Miklós Gaszner,

C. David Allis,

Gary Felsenfeld

Publication year - 2001

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1064413

Subject(s) - histone , histone h3 , chromatin , acetylation , chromatin immunoprecipitation , methylation , biology , histone methylation , histone h1 , locus (genetics) , lysine , microbiology and biotechnology , dna methylation , genetics , gene , promoter , gene expression , amino acid

Methylation of histones at specific residues plays an important role in transcriptional regulation. Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta-globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation. Lysine 9 is methylated most over constitutive condensed chromatin and developmentally inactive globin genes. In contrast, lysine 4 methylation of histone H3 correlates with H3 acetylation. These results lead us to propose a mechanism by which the insulator in the beta-globin locus can protect the globin genes from being silenced by adjacent condensed chromatin.

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