Synaptotagmin Modulation of Fusion Pore Kinetics in Regulated Exocytosis of Dense-Core Vesicles | Zendy

ChihTien Wang | Zendy; Ruslan N. Grishanin | Zendy; Cynthia A. Earles | Zendy; Payne Y. Chang | Zendy; Thomas F.J. Martin | Zendy; Edwin R. Chapman | Zendy; Meyer B. Jackson | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Synaptotagmin Modulation of Fusion Pore Kinetics in Regulated Exocytosis of Dense-Core Vesicles

Author(s) -

ChihTien Wang,

Ruslan N. Grishanin,

Cynthia A. Earles,

Payne Y. Chang,

Thomas F.J. Martin,

Edwin R. Chapman,

Meyer B. Jackson

Publication year - 2001

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1064002

Subject(s) - synaptotagmin 1 , exocytosis , vesicle , vesicle fusion , chemistry , biophysics , fusion , lipid bilayer fusion , synaptotagmin i , microbiology and biotechnology , stx1a , membrane , biochemistry , synaptic vesicle , syntaxin , biology , linguistics , philosophy

In the exocytosis of neurotransmitter, fusion pore opening represents the first instant of fluid contact between the vesicle lumen and extracellular space. The existence of the fusion pore has been established by electrical measurements, but its molecular composition is unknown. The possibility that synaptotagmin regulates fusion pores was investigated with amperometry to monitor exocytosis of single dense-core vesicles. Overexpression of synaptotagmin I prolonged the time from fusion pore opening to dilation, whereas synaptotagmin IV shortened this time. Both synaptotagmin isoforms reduced norepinephrine flux through open fusion pores. Thus, synaptotagmin interacts with fusion pores, possibly by associating with a core complex of membrane proteins and/or lipid.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research