A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegans | Zendy

Scott W. Knight | Zendy; Brenda Bass | Zendy

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A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegans

Author(s) -

Scott W. Knight,

Brenda Bass

Publication year - 2001

Publication title -

science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 12.556

H-Index - 1186

eISSN - 1095-9203

pISSN - 0036-8075

DOI - 10.1126/science.1062039

Subject(s) - rna interference , dicer , rna silencing , ribonuclease iii , biology , caenorhabditis elegans , rna , rnase p , mutant , genetics , cleavage (geology) , gene , microbiology and biotechnology , paleontology , fracture (geology)

An early event in RNA interference (RNAi) is the cleavage of the initiating double-stranded RNA (dsRNA) to short pieces, 21 to 23 nucleotides in length. Here we describe a null mutation in dicer-1 (dcr-1), a gene proposed to encode the enzyme that generates these short RNAs. We find that dcr-1(-/-) animals have defects in RNAi under some, but not all, conditions. Mutant animals have germ line defects that lead to sterility, suggesting that cleavage of dsRNA to short pieces is a requisite event in normal development.

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