Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination | Zendy

Yan Kuai | Zendy; Junxue Chen | Zendy; Xi Tang | Zendy; Yifeng Xiang | Zendy; Fengya Lu | Zendy; Cuifang Kuang | Zendy; Liang Xu | Zendy; Weidong Shen | Zendy; Junjie Cheng | Zendy; Huaqiao Gui | Zendy; Gang Zou | Zendy; Pei Wang | Zendy; Hai Ming | Zendy; Jianguo Liu | Zendy; Xü Liu | Zendy; Joseph R. Lakowicz | Zendy; Douguo Zhang | Zendy

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Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination

Author(s) -

Yan Kuai,

Junxue Chen,

Xi Tang,

Yifeng Xiang,

Fengya Lu,

Cuifang Kuang,

Liang Xu,

Weidong Shen,

Junjie Cheng,

Huaqiao Gui,

Gang Zou,

Pei Wang,

Hai Ming,

Jianguo Liu,

Xü Liu,

Joseph R. Lakowicz,

Douguo Zhang

Publication year - 2019

Publication title -

science advances

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.928

H-Index - 146

ISSN - 2375-2548

DOI - 10.1126/sciadv.aav5335

Subject(s) - microscopy , resolution (logic) , rotation (mathematics) , azimuth , optics , photonics , materials science , surface (topology) , image resolution , super resolution microscopy , scanning confocal electron microscopy , physics , computer science , computer vision , artificial intelligence , geometry , mathematics

Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology.

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