BioBits™ Bright: A fluorescent synthetic biology education kit | Zendy

Jessica C. Stark | Zendy; Ally Huang | Zendy; Peter Q. Nguyen | Zendy; Rachel S. Dubner | Zendy; Karen J. Hsu | Zendy; Thomas C. Ferrante | Zendy; Mary Anderson | Zendy; Ada Kanapskyte | Zendy; Quinn Mucha | Zendy; Jessica S. Packett | Zendy; Palak Patel | Zendy; Richa Patel | Zendy; Deema Qaq | Zendy; Tyler Zondor | Zendy; Julie A. Burke | Zendy; Thomas Martinez | Zendy; Ashlee Miller-Berry | Zendy; Aparna Puppala | Zendy; Kara Reichert | Zendy; Miriam Schmid | Zendy; Lance Brand | Zendy; Lander R. Hill | Zendy; Jemima F. Chellaswamy | Zendy; Nuhie Faheem | Zendy; Suzanne Fetherling | Zendy; Elissa Gong | Zendy; Eddie Marie Gonzalzles | Zendy; Teresa Granito | Zendy; Jenna Koritsaris | Zendy; Hoa Binh Nguyen | Zendy; Sujud Ottman | Zendy; Christina Palffy | Zendy; Angela Patel | Zendy; Sheila Skweres | Zendy; Adriane Slaton | Zendy; TaRhonda Woods | Zendy; Nina M. Donghia | Zendy; Keith Pardee | Zendy; James J. Collins | Zendy; Michael C. Jewett | Zendy

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BioBits™ Bright: A fluorescent synthetic biology education kit

Author(s) -

Jessica C. Stark,

Ally Huang,

Peter Q. Nguyen,

Rachel S. Dubner,

Karen J. Hsu,

Thomas C. Ferrante,

Mary Anderson,

Ada Kanapskyte,

Quinn Mucha,

Jessica S. Packett,

Palak Patel,

Richa Patel,

Deema Qaq,

Tyler Zondor,

Julie A. Burke,

Thomas Martinez,

Ashlee Miller-Berry,

Aparna Puppala,

Kara Reichert,

Miriam Schmid,

Lance Brand,

Lander R. Hill,

Jemima F. Chellaswamy,

Nuhie Faheem,

Suzanne Fetherling,

Elissa Gong,

Eddie Marie Gonzalzles,

Teresa Granito,

Jenna Koritsaris,

Hoa Binh Nguyen,

Sujud Ottman,

Christina Palffy,

Angela Patel,

Sheila Skweres,

Adriane Slaton,

TaRhonda Woods,

Nina M. Donghia,

Keith Pardee,

James J. Collins,

Michael C. Jewett

Publication year - 2018

Publication title -

science advances

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.928

H-Index - 146

ISSN - 2375-2548

DOI - 10.1126/sciadv.aat5107

Subject(s) - fluorescence , computational biology , biology , physics , optics

Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits™ Bright, a shelf-stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing

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