Open AccessA conformational checkpoint between DNA binding and cleavage by CRISPR-Cas9
Author(s) -
Yavuz S. Dagdas,
Janice S. Chen,
Samuel H. Sternberg,
Jennifer A. Doudna,
Ahmet Yıldız
Publication year - 2017
Publication title -
science advances
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.928
H-Index - 146
ISSN - 2375-2548
DOI - 10.1126/sciadv.aao0027
Subject(s) - crispr , cleavage (geology) , cas9 , dna , g2 m dna damage checkpoint , microbiology and biotechnology , chemistry , computational biology , biophysics , biology , cell cycle checkpoint , biochemistry , cell cycle , apoptosis , gene , paleontology , fracture (geology)
A conformational checkpoint of Cas9 enables on-target cleavage while blocking access to the active state at off-target sites.
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