Light-activated communication in synthetic tissues | Zendy

Michael J. Booth | Zendy; Vanessa Restrepo Schild | Zendy; Alexander Graham | Zendy; Sam N. Olof | Zendy; Hagan Bayley | Zendy

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Light-activated communication in synthetic tissues

Author(s) -

Michael J. Booth,

Vanessa Restrepo Schild,

Alexander Graham,

Sam N. Olof,

Hagan Bayley

Publication year - 2016

Publication title -

science advances

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.928

H-Index - 146

ISSN - 2375-2548

DOI - 10.1126/sciadv.1600056

Subject(s) - 3d printed , translation (biology) , dna , bilayer , microbiology and biotechnology , synthetic biology , transcription factor , nanotechnology , in vitro , biophysics , materials science , chemistry , gene , biology , computational biology , membrane , biochemistry , messenger rna , biomedical engineering , medicine

We have previously used three-dimensional (3D) printing to prepare tissue-like materials in which picoliter aqueous compartments are separated by lipid bilayers. These printed droplets are elaborated into synthetic cells by using a tightly regulated in vitro transcription/translation system. A light-activated DNA promoter has been developed that can be used to turn on the expression of any gene within the synthetic cells. We used light activation to express protein pores in 3D-printed patterns within synthetic tissues. The pores are incorporated into specific bilayer interfaces and thereby mediate rapid, directional electrical communication between subsets of cells. Accordingly, we have developed a functional mimic of neuronal transmission that can be controlled in a precise way

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