Size effect of complexed plasmid DNA to gene transfection efficiency of microbubble-mediated sonoporation | Zendy

Yoichiro Matsumoto | Zendy; Yiwei Zhang | Zendy; Takashi Azuma | Zendy; Kiyoshi Yoshinaka | Zendy; Kensuke Osada | Zendy; Kazunori Kataoka | Zendy; Shu Takagi | Zendy

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Size effect of complexed plasmid DNA to gene transfection efficiency of microbubble-mediated sonoporation

Author(s) -

Yoichiro Matsumoto,

Yiwei Zhang,

Takashi Azuma,

Kiyoshi Yoshinaka,

Kensuke Osada,

Kazunori Kataoka,

Shu Takagi

Publication year - 2013

Publication title -

proceedings of meetings on acoustics

Language(s) - English

Resource type - Conference proceedings

ISSN - 1939-800X

DOI - 10.1121/1.4800339

Subject(s) - sonoporation , transfection , microbubbles , biophysics , gene delivery , micelle , fluorescence , plasmid , green fluorescent protein , dna , chemistry , naked dna , dynamic light scattering , materials science , microbiology and biotechnology , nanoparticle , nanotechnology , gene , ultrasound , optics , biology , biochemistry , physics , aqueous solution , acoustics

Ultrasound-mediated gene transfection in the presence of microbubbles is a recently developed, promising nonviral gene delivery method. Detailed dynamics of pore opening on the cell surface have not been clarified. In particular, pore size is one of the most essential parameters. In this study, we investigated the size effect of complexed plasmid DNA (pDNA) on transfection efficiency by packaging the pDNA within polyplex micelles. Both naked pDNA and complexed pDNA were transfected into cultured NIH3T3 cells by using ultrasound in the presence of a microbubble contrast agent, Sonazoid. The sizes of the hydrodynamic diameter of the naked and complexed pDNA, estimated by a dynamic light scattering measurement, were 600 and 120 nm, respectively. The transfection rates of the complexed pDNA, evaluated by counting the number of cells that exhibited green fluorescence, was 1.67%, while that of the naked pDNA was 0.92%. This efficiency enhancement, which depends on size reduction, showed that the pore sizes were distributed in the range of the pDNA diameters.

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