Position-referenced microscopy: regions of interest localization and subpixel image comparison by means of pseudo-random patterns embedded in cell culture boxes | Zendy

July A. Galeano Zea | Zendy; Patrick Sandoz | Zendy; Laurent Robert | Zendy; Emilie Gaiffe | Zendy; JeanLuc Prétet | Zendy; Christiane Mougin | Zendy

Open Access

Position-referenced microscopy: regions of interest localization and subpixel image comparison by means of pseudo-random patterns embedded in cell culture boxes

Author(s) -

July A. Galeano Zea,

Patrick Sandoz,

Laurent Robert,

Emilie Gaiffe,

JeanLuc Prétet,

Christiane Mougin

Publication year - 2009

Publication title -

proceedings of spie, the international society for optical engineering/proceedings of spie

Language(s) - English

Resource type - Conference proceedings

SCImago Journal Rank - 0.192

H-Index - 176

eISSN - 1996-756X

pISSN - 0277-786X

DOI - 10.1117/12.831538

Subject(s) - subpixel rendering , superimposition , computer science , position (finance) , computer vision , pixel , artificial intelligence , microscopy , optics , physics , finance , economics

International audienceThis work presents micro-grids integrated to cell culture boxes. These grids allow the systematic registration of the position of a zone observed by optical microscopy in a such way that it is possible to find it again easily for new observations for instance after culture on drug injection. The position knowledge allows also the numerical superimposition of recorded images in a common position reference system with a sub-pixel precision. It become thus straightforward to perform a site by site analysis of the possible evolutions that may have occurred in the biological medium

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