In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy | Zendy

Wen Lo | Zendy; Yuh-Ling Chang | Zendy; Yen Sun | Zendy; SungJan Lin | Zendy; ShiouHwa Jee | Zendy; HsinYuan Tan | Zendy; ChenYuan Dong | Zendy

Open Access

In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy

Author(s) -

Wen Lo,

Yuh-Ling Chang,

Yen Sun,

SungJan Lin,

ShiouHwa Jee,

HsinYuan Tan,

ChenYuan Dong

Publication year - 2007

Publication title -

proceedings of spie, the international society for optical engineering/proceedings of spie

Language(s) - English

Resource type - Conference proceedings

SCImago Journal Rank - 0.192

H-Index - 176

eISSN - 1996-756X

pISSN - 0277-786X

DOI - 10.1117/12.699709

Subject(s) - autofluorescence , cornea , wound healing , biomedical engineering , organ culture , microscopy , in vivo , visualization , in vitro , regeneration (biology) , pathology , confocal microscopy , materials science , medicine , optics , chemistry , microbiology and biotechnology , ophthalmology , computer science , fluorescence , biology , surgery , artificial intelligence , physics , biochemistry

The aim of this work is to image the wound healing process of cornea in an in vitro organ culture model with noninvasive multiphoton imaging modality. Autofluorescence and second harmonic generation (SHG) were respectively used to monitor the alterations of cellular and collagenous components during wound healing processes. Within additional developments, this approach may be applied to in vivo visualization of corneal structural destruction and the subsequent regeneration.

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