Open AccessImaging of skin dermal thermal damage by multiphoton autofluroescence and second harmonic generation (SHG) microscopy
Author(s) -
TsungLin Yang,
ChengTien Chiang,
Hsien-Ching Kao,
Wen Lo,
SungJan Lin,
ShiouHwa Jee,
YangFang Chen,
ChenYuan Dong
Publication year - 2006
Publication title -
proceedings of spie, the international society for optical engineering/proceedings of spie
Language(s) - English
Resource type - Conference proceedings
SCImago Journal Rank - 0.192
H-Index - 176
eISSN - 1996-756X
pISSN - 0277-786X
DOI - 10.1117/12.647406
Subject(s) - elastin , autofluorescence , dermis , materials science , microscopy , second harmonic generation , optical microscope , multiphoton fluorescence microscope , thermal burn , optics , biomedical engineering , laser , fluorescence microscope , scanning electron microscope , pathology , composite material , fluorescence , medicine , physics
Multiphoton autofluorescence and second harmonic generation (SHG) microscopy are useful in respectively identifying elastin and collagen fibers within the skin dermis. In this study, we attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize the thermal changes to collagen and elastin fibers. We found that autofluorescence and SHG imaging behave differently in skin dermis treated with different temperatures and that an index reflecting the relative changes in autofluorescence and SHG intensity is useful to identify the degree of dermal thermal damage to the skin. With additional development, our approach can be used to identify the extent of thermal damage in patients.
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