Local amplitude and phase retrieval method for digital holography applied to microscopy | Zendy

Michael Liebling | Zendy; Thierry Blu | Zendy; Etienne Cuche | Zendy; Pierre Marquet | Zendy; Christian Depeursinge | Zendy; Michaël Unser | Zendy

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Local amplitude and phase retrieval method for digital holography applied to microscopy

Author(s) -

Michael Liebling,

Thierry Blu,

Etienne Cuche,

Pierre Marquet,

Christian Depeursinge,

Michaël Unser

Publication year - 2003

Publication title -

proceedings of spie, the international society for optical engineering/proceedings of spie

Language(s) - English

Resource type - Conference proceedings

SCImago Journal Rank - 0.192

H-Index - 176

eISSN - 1996-756X

pISSN - 0277-786X

DOI - 10.1117/12.500517

Subject(s) - digital holographic microscopy , digital holography , holography , computer science , phase retrieval , focus (optics) , optics , amplitude , phase (matter) , microscopy , plane (geometry) , fresnel diffraction , cardinal point , computer vision , algorithm , fourier transform , physics , diffraction , mathematics , geometry , quantum mechanics

We present a numerical two-step reconstruction procedure for digital off-axis Fresnel holograms. First, we retrieve t he amplitude and phase of the object wave in the CCD plane. For each point we solve a weighted linear set of equations in the least-squares sense. The algorithm has O(N) complexity and gives great flexibility. Second, we numerica lly propagate the obtained wave to achieve proper focus. We apply the method to microscopy and demonstrate its suitability for the real time imaging of biological samples.

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