DHMI: dynamic holographic microscopy interface | Zendy

Xuefei He | Zendy; Yujie Zheng | Zendy; Woei Ming Lee | Zendy

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DHMI: dynamic holographic microscopy interface

Author(s) -

Xuefei He,

Yujie Zheng,

Woei Ming Lee

Publication year - 2016

Publication title -

proceedings of spie, the international society for optical engineering/proceedings of spie

Language(s) - English

Resource type - Conference proceedings

SCImago Journal Rank - 0.192

H-Index - 176

eISSN - 1996-756X

pISSN - 0277-786X

DOI - 10.1117/12.2242948

Subject(s) - holography , digital holographic microscopy , microscopy , computer science , interface (matter) , computer graphics (images) , materials science , optics , physics , bubble , maximum bubble pressure method , parallel computing

Digital holographic microscopy (DHM) is a powerful in-vitro biological imaging tool. In this paper, we report a fully automated off-axis digital holographic microscopy system completed with a graphical user interface in the Matlab © environment. The interface primarily includes Fourier domain processing, phase reconstruction, aberration compensation and autofocusing. A variety of imaging operations such as region of interest selection, de-noising mode (filtering and averaging), low frame rate imaging for immediate reconstruction and high frame rate imaging routine (~ 27 fps) are implemented to facilitate ease of use.

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