Adaptive spatial filtering for off-axis digital holographic microscopy based on region recognition approach with iterative thresholding | Zendy

Xuefei He | Zendy; Chuong Nguyen | Zendy; Mrinalini Pratap | Zendy; Yujie Zheng | Zendy; Yi Wang | Zendy; David R. Nisbet | Zendy; Melanie Rug | Zendy; Alexander G. Maier | Zendy; Woei Lee | Zendy

Open Access

Adaptive spatial filtering for off-axis digital holographic microscopy based on region recognition approach with iterative thresholding

Author(s) -

Xuefei He,

Chuong Nguyen,

Mrinalini Pratap,

Yujie Zheng,

Yi Wang,

David R. Nisbet,

Melanie Rug,

Alexander G. Maier,

Woei Lee

Publication year - 2016

Publication title -

proceedings of spie, the international society for optical engineering/proceedings of spie

Language(s) - English

Resource type - Conference proceedings

SCImago Journal Rank - 0.192

H-Index - 176

eISSN - 1996-756X

pISSN - 0277-786X

DOI - 10.1117/12.2242876

Subject(s) - thresholding , artificial intelligence , computer science , computer vision , digital holographic microscopy , holography , pattern recognition (psychology) , iterative method , spatial filter , image resolution , microscopy , image (mathematics) , algorithm , optics , physics

Here we propose a region-recognition approach with iterative thresholding, which is adaptively tailored to extract the appropriate region or shape of spatial frequency. In order to justify the method, we tested it with different samples and imaging conditions (different objectives). We demonstrate that our method provides a useful method for rapid imaging of cellular dynamics in microfluidic and cell cultures.

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