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The detection and quantification of nanoparticles is a complex issue due to the need to combine “classical” identification and quantification of the constituent material, with the accurate determination of the size of sub-micrometer objects, usually well below the optical diffraction limit. In this work we show that one of the most used analytical methods for silver nanoparticles, asymmetric flow field-flow fractionation, can be strongly influenced by the presence of dissolved organic matter (such as alginate) and lead to potentially misleading results. We explain the anomalies in the separation process and we show a very general way forward based on the combination of size separation and size measurement techniques. This combination of techniques results in more robust AF4-based methods for the sizing of silver nanoparticles in environmental conditions and could be generally applied to the sizing of nanoparticles in complex matrices.JRC.F.2-Consumer Products Safet

Characterization of silver nanoparticles-alginate complexes by combined size separation and size measurement techniques