Combined microslit electrokinetic measurements and reflectometric interference spectroscopy to study protein adsorption processes | Zendy

Ralf Zimmermanna | Zendy; Toshihisa Osaki | Zendy; Günter Gauglitz | Zendy; Carsten Werner | Zendy

Open Access

Combined microslit electrokinetic measurements and reflectometric interference spectroscopy to study protein adsorption processes

Author(s) -

Ralf Zimmermanna,

Toshihisa Osaki,

Günter Gauglitz,

Carsten Werner

Publication year - 2007

Publication title -

biointerphases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.633

H-Index - 45

eISSN - 1934-8630

pISSN - 1559-4106

DOI - 10.1116/1.2814066

Subject(s) - electrokinetic phenomena , adsorption , desorption , chemistry , spectroscopy , analytical chemistry (journal) , polymer , chemical engineering , maleic acid , interference (communication) , characterization (materials science) , chemical physics , materials science , nanotechnology , chromatography , copolymer , organic chemistry , channel (broadcasting) , physics , electrical engineering , quantum mechanics , engineering

Streaming potential/current measurements for the characterization of charge formation processes at solid/liquid interfaces were combined with reflectometric interference spectroscopy. The simultaneous determination of electrosurface characteristics and the optical thickness of interfacial layers provides information on structural variations of adsorbed or covalently bound polymers and on charge dependent adsorption and desorption phenomena at solid/liquid interfaces. To demonstrate the potentialities of this extended approach for biointerfacial studies the authors report a series of experiments on the adsorption of the plasma protein fibrinogen at poly(octadecene-alt-maleic acid) thin films.

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