Open AccessIntegration of cytochrome c oxidase I barcodes and geometric morphometrics to delimit species in the genus G nopharmia ( L epidoptera: G eometridae, E nnominae)
Author(s) -
Rajaei Sh Hossein,
Struwe JanFrederic,
Raupach Michael J.,
Ahrens Dirk,
Wägele J. Wolfgang
Publication year - 2013
Publication title -
zoological journal of the linnean society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.148
H-Index - 83
eISSN - 1096-3642
pISSN - 0024-4082
DOI - 10.1111/zoj.12053
Subject(s) - biology , morphometrics , dna barcoding , aedeagus , taxonomy (biology) , zoology , cytochrome c oxidase subunit i , mitochondrial dna , evolutionary biology , genus , genetics , gene
We tested the hypothesis of species taxonomy in the genus G nopharmia ( M acariini, E nnominae) that was recently established in a review based on discrete morphological characters. For this objective we integrated both DNA ‐based and morphometric approaches in order to infer species boundaries. A 658‐bp fragment of the mitochondrial cytochrome c oxidase subunit 1 ( CO1 ) ( DNA barcode) was analysed from populations of five species distributed throughout the M iddle E ast to assess their consistency with traditionally defined morphospecies. Signals in the morphological variation of the aedeagus of all relevant populations were evaluated using geometric landmarks. Consistent groupings compatible with the current taxonomic classification were found with both approaches. The results strongly support the distinction of seven closely related species. © 2013 The Linnean Society of London