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Current methods to analyze lysosome morphology, positioning, motility and function
Author(s) -
Barral Duarte C.,
Staiano Leopoldo,
Guimas Almeida Cláudia,
Cutler Dan F.,
Eden Emily R.,
Futter Clare E.,
Galione Antony,
Marques André R. A.,
Medina Diego Luis,
Napolitano Gennaro,
Settembre Carmine,
Vieira Otília V.,
Aerts Johannes M. F. G.,
AtakpaAdaji Peace,
Bruno Gemma,
Capuozzo Antonella,
De Leonibus Elvira,
Di Malta Chiara,
Escrevente Cristina,
Esposito Alessandra,
Grumati Paolo,
Hall Michael J.,
Teodoro Rita O.,
Lopes Susana S.,
Luzio J. Paul,
Monfregola Jlenia,
Montefusco Sandro,
Platt Frances M.,
Polishchuck Roman,
De Risi Maria,
Sambri Irene,
Soldati Chiara,
Seabra Miguel C.
Publication year - 2022
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/tra.12839
Subject(s) - lysosome , organelle , biology , function (biology) , microbiology and biotechnology , motility , computational biology , biochemistry , enzyme
Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has shown that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyze lysosome morphology, positioning, motility and function. We highlight the principles behind these methods, the methodological strategies and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground‐breaking discoveries on an organelle that continues to surprise and excite cell biologists.