Open AccessIdentification of a UDP ‐glucosyltransferase conferring deoxynivalenol resistance in Aegilops tauschii and wheat
Author(s) -
Kirana Rizky Pasthika,
Gaurav Kumar,
Arora Sanu,
Wiesenberger Gerlinde,
Doppler Maria,
Michel Sebastian,
Zimmerl Simone,
Matic Magdalena,
Eze Chinedu E.,
Kumar Mukesh,
Topuz Ajla,
Lemmens Marc,
Schuhmacher Rainer,
Adam Gerhard,
Wulff Brande B. H.,
Buerstmayr Hermann,
Steiner Barbara
Publication year - 2023
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/pbi.13928
Subject(s) - aegilops tauschii , biology , genetics , gene , fusarium , ploidy , allele , aegilops , chromosome
Summary Aegilops tauschii is the diploid progenitor of the wheat D subgenome and a valuable resource for wheat breeding, yet, genetic analysis of resistance against Fusarium head blight (FHB) and the major Fusarium mycotoxin deoxynivalenol (DON) is lacking. We treated a panel of 147 Ae. tauschii accessions with either Fusarium graminearum spores or DON solution and recorded the associated disease spread or toxin‐induced bleaching. A k ‐mer‐based association mapping pipeline dissected the genetic basis of resistance and identified candidate genes. After DON infiltration nine accessions revealed severe bleaching symptoms concomitant with lower conversion rates of DON into the non‐toxic DON‐3‐O‐glucoside. We identified the gene AET5Gv20385300 on chromosome 5D encoding a uridine diphosphate (UDP)‐glucosyltransferase (UGT) as the causal variant and the mutant allele resulting in a truncated protein was only found in the nine susceptible accessions. This UGT is also polymorphic in hexaploid wheat and when expressed in Saccharomyces cerevisiae only the full‐length gene conferred resistance against DON. Analysing the D subgenome helped to elucidate the genetic control of FHB resistance and identified a UGT involved in DON detoxification in Ae. tauschii and hexaploid wheat. This resistance mechanism is highly conserved since the UGT is orthologous to the barley UGT HvUGT13248 indicating descent from a common ancestor of wheat and barley.