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N471D WASH complex subunit strumpellin knock‐in mice display mild motor and cardiac abnormalities and BPTF and KLHL11 dysregulation in brain tissue
Author(s) -
Clemen Christoph S.,
Schmidt Andreas,
Winter Lilli,
Canneva Fabio,
Wittig Ilka,
Becker Lore,
Coras Roland,
Berwanger Carolin,
Hofmann Andreas,
Eggers Britta,
Marcus Katrin,
GailusDurner Valerie,
Fuchs Helmut,
Angelis Martin Hrabe,
Krüger Marcus,
Hörsten Stephan,
Eichinger Ludwig,
Schröder Rolf
Publication year - 2022
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/nan.12750
Subject(s) - downregulation and upregulation , knockout mouse , heterozygote advantage , biology , protein subunit , neurodegeneration , haploinsufficiency , endocrinology , compound heterozygosity , phenotype , medicine , pathology , genetics , gene , allele , disease
Aims We investigated N471D WASH complex subunit strumpellin ( Washc5 ) knock‐in and Washc5 knock‐out mice as models for hereditary spastic paraplegia type 8 (SPG8). Methods We generated heterozygous and homozygous N471D Washc5 knock‐in mice and subjected them to a comprehensive clinical, morphological and laboratory parameter screen, and gait analyses. Brain tissue was used for proteomic analysis. Furthermore, we generated heterozygous Washc5 knock‐out mice. WASH complex subunit strumpellin expression was determined by qPCR and immunoblotting. Results Homozygous N471D Washc5 knock‐in mice showed mild dilated cardiomyopathy, decreased acoustic startle reactivity, thinner eye lenses, increased alkaline phosphatase and potassium levels and increased white blood cell counts. Gait analyses revealed multiple aberrations indicative of locomotor instability. Similarly, the clinical chemistry, haematology and gait parameters of heterozygous mice also deviated from the values expected for healthy animals, albeit to a lesser extent. Proteomic analysis of brain tissue depicted consistent upregulation of BPTF and downregulation of KLHL11 in heterozygous and homozygous knock‐in mice. WASHC5‐related protein interaction partners and complexes showed no change in abundancies. Heterozygous Washc5 knock‐out mice showing normal WASHC5 levels could not be bred to homozygosity. Conclusions While biallelic ablation of Washc5 was prenatally lethal, expression of N471D mutated WASHC5 led to several mild clinical and laboratory parameter abnormalities, but not to a typical SPG8 phenotype. The consistent upregulation of BPTF and downregulation of KLHL11 suggest mechanistic links between the expression of N471D mutated WASHC5 and the roles of both proteins in neurodegeneration and protein quality control, respectively.