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LncRNA LOXL1‐AS1 inhibits proliferation of PDLSCs and downregulates IL‐1β in periodontitis patients
Author(s) -
Ruan Danping,
Wu Chunyun,
Zhang Yu,
Zhang Yu
Publication year - 2022
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1111/jre.12962
Subject(s) - periodontitis , downregulation and upregulation , gene knockdown , medicine , cancer research , immunology , biology , apoptosis , gene , genetics
Background The carcinogenic role of lncRNA LOXL1‐AS1 in thoracic aortic aneurysm has been confirmed, but its role in the pathogenesis of periodontitis remains unclear. Our preliminary deep sequencing data revealed LOXL1‐AS1 downregulation in periodontitis and its inverse correlation with IL‐1β, a critical inflammatory mediator in periodontitis. This study was therefore performed to investigate the potential interaction between LOXL1‐AS1 and IL‐1β in periodontitis. Methods The study included 30 periodontitis patients (18 males and 12 females at the age of 34 to 44 years, with a mean of 39.3 ± 2.1 years) and 30 healthy controls (18 males and 12 females at the age of 33 to 44 years with a mean of 39.2 ± 2.0 years). The effects of LOXL1‐AS1 overexpression on IL‐1β were evaluated by RT‐qPCR and Western blot. CCK‐8 assay was used to analyze cell proliferation. Results LOXL1‐AS1 was downregulated in periodontitis‐affected periodontal ligament stem cells (PDLSCs) compared with healthy PDLSCs, while IL‐1β was upregulated in periodontitis‐affected PDLSCs and was inversely correlated with LOXL1‐AS1. LOXL1‐AS1 overexpression mediated IL‐1β downregulation in PDLSCs. IL‐1β treatment did not affect LOXL1‐AS1 expression. Moreover, LOXL1‐AS1 overexpression inhibited the proliferation of periodontitis‐affected PDLSCs. LOXL1‐AS1 overexpression and IL‐1β knockdown increased Bax/Bcl‐2 ratio and caspase‐3 level. Conclusion This study is the first to report LOXL1‐AS1 downregulation in periodontitis. Moreover, LOXL1‐AS1 might inhibit the proliferation of periodontitis‐affected PDLSCs and downregulate IL‐1β to improve periodontitis.