Open AccessTherapeutic effects of Qianlie Tongli decoction on chronic prostatitis/chronic pelvic pain syndrome induced by peptide T2 in mice
Author(s) -
Cui Xingxing,
Naveed Muhammad,
Baig Mirza Muhammad Faran Ashraf,
Wang Wenlu,
Mikrani Reyaj,
Liu Ziwei,
Ahmad Bilal,
Tang Meng,
Wazir Junaid,
Zhou Xiaohui,
Han Lei
Publication year - 2020
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1111/jphp.13325
Subject(s) - medicine , prostatitis , decoction , tripterygium wilfordii , traditional chinese medicine , prostate , therapeutic effect , saline , urinary system , pelvic pain , inflammation , urine , urology , pathology , surgery , alternative medicine , cancer
Objectives This study was undertaken to reveal therapeutic effects and the preliminary mechanism of Chinese medicine formula Qianlie Tongli decoction (QTD) in chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). Methods A total of 50 male C57BL/6 mice were randomly divided into five groups. All groups except the control group were injected subcutaneously T2 peptide emulsion, which induced the CP/CPPS model. After the induction of CP/CPPS, the model group was given normal saline by oral gavage while low‐dose, medium‐dose and high‐dose groups were treated with Chinese medicine formula. Micturition habits and pain behaviour of mice were analysed for each group. Haematoxylin and eosin (H&E) staining was used to investigate prostate inflammation. The serum level of tumour necrosis factor‐α (TNF‐α) was measured by enzyme‐linked immunosorbent assay (ELISA) kit. Key findings Chinese medicine formula significantly reduced the number of urine spots and improved pain response frequency in the medium‐dose and high‐dose group. The high‐dose group showed reduced considerably inflammatory lesion and inflammatory cell infiltration than the low‐dose and medium‐dose groups. Serum levels of TNF‐α in the high‐dose group were significantly reduced compared with the model group. Conclusions The results demonstrated the therapeutic effects of Qianlie Tongli decoction in CP/CPPS mice by analysing clinically relevant symptoms (urinary tract system, pelvic pain and prostate inflammation) and preliminarily explored the inflammatory‐related treatment mechanisms by measuring TNF‐α.