Open AccessBy reducing oxidative stress, naringenin mitigates hyperglycaemia‐induced upregulation of hepatic nuclear factor erythroid 2‐related factor 2 protein
Author(s) -
Kometsi Lereko,
Govender Khmera,
Mofo Mato Edith P.,
Hurchund Rajendraparsad,
Owira Peter M. O.
Publication year - 2020
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1111/jphp.13319
Subject(s) - naringenin , oxidative stress , antioxidant , chemistry , endocrinology , streptozotocin , medicine , downregulation and upregulation , biochemistry , diabetes mellitus , flavonoid , gene
Objectives Antioxidant and anti‐inflammatory properties of naringenin could confer hepatoprotective effects. Methods Chang cells in culture media were maintained at 37°C and treated with increased concentrations of glucose (5.5–50 m m ) and/or naringenin (25–100 µ m ), respectively, for 24 h. The cells were harvested and carbonyl proteins, antioxidant enzymes and proteins measured in cell lysates. Sprague Dawley rats were divided into 5 groups ( n = 7) and orally treated daily for 56 days with 3.0 ml/kg per body weight (BW) distilled water (group 1), 60 mg/kg BW of naringenin (groups 2 and 4), respectively. Groups 3, 4 and 5 were given single 60 mg/kg per BW intraperitoneal injections of streptozotocin or insulin (2.0 IU/kg BW bid), (group 5 only). Key findings Cell viability was significantly decreased in response to increased hyperglycaemia but naringenin dose‐dependently, significantly reversed this compared to controls, respectively. However, antioxidant enzyme activities were reduced due to increased and reduced oxidative stress, respectively. Naringenin further significantly reduced hepatic oxidative stress and nuclear factor erythroid 2‐related factor 2 (Nrf2) protein expression and liver : body weight ratios in diabetic compared to controls rats. Conclusions Naringenin confers hepatoprotective antioxidant effects by initially preventing upregulation of Nrf2 protein expression and its downstream antioxidant enzymes.