Open AccessAntihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice
Author(s) -
Xu Wenhao,
Wang Hantao,
Sun Ying,
Xue Zhencheng,
Liang Mingli,
Su Weike
Publication year - 2020
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1111/jphp.13222
Subject(s) - hyperuricemia , xanthine oxidase , allopurinol , pharmacology , chemistry , kidney , traditional medicine , ethyl acetate , uric acid , biochemistry , medicine , enzyme
Objectives To investigate the antihyperuricemia and nephroprotective effects of Orthosiphon stamineus extracts on hyperuricemia (HUA) mice and explore the potential mechanisms. Methods Orthosiphon stamineus extracts were extracted using 50% ethanol and enriched using ethyl acetate, and characterised utilising UPLC/ESI‐MS. A potassium oxonate (PO) induced hyperuricemic mouse model was used to evaluate antihyperuricemia and nephroprotective effects of O. stamineus ethyl acetate extracts (OSE). Key findings Eight constituents from OSE were identified and OSE treatment ameliorated HUA by regulating key indicators of kidney dysfunction and xanthine oxidase, adenosine deaminase activity and urate transporters in hyperuricemic mice. Moreover, in renal histopathology analysis, OSE significantly alleviated kidney injury. Conclusions These findings demonstrate that OSE has antihyperuricemic and nephroprotective effects on PO‐induced HUA mice and those results indicate that OSE could be a safe and effective agent or functional ingredient for treating HUA.