Open AccessPhospholipase Cγ2 signalling contributes to the haemostatic effect of Notoginsenoside Ft1
Author(s) -
Liu Yingqiu,
Liu Tianyi,
Zhao Jing,
He Taotao,
Chen Hua,
Wang Jiaqing,
Zhang Weimin,
Ma Wuren,
Fan Yunpeng,
Song Xiaoping
Publication year - 2019
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1111/jphp.13057
Subject(s) - panax notoginseng , platelet , pharmacology , long term potentiation , thrombin , phospholipase c , chemistry , aspirin , platelet aggregation , medicine , signal transduction , receptor , biochemistry , alternative medicine , pathology
Objectives The drawback of bleeding caused by chronic antiplatelet therapy is persecuting patients with thrombotic diseases severely. Based on the dual‐directional regulatory effect of Panax notoginseng on platelet, the present study focused on the effect of Notoginsenoside Ft1, a saponin with effect in promoting platelet aggregation. Key findings According to the present study, Notoginsenoside Ft1 cannot stimulate platelet aggregation independently. However, the effect in enhancing aggregation induced by thrombin, collagen and ADP is peaked at 5–10 μ m . In addition, thrombin‐induced activation of PLC γ2‐ IP 3 / DAG ‐[Ca 2+ ]/ PKC ‐ TXA 2 signalling was potentiated by Notoginsenoside Ft1, as well. Furthermore, the mice tail bleeding time was shortened by administration of Notoginsenoside Ft1 significantly. And the bleeding time prolonged by aspirin was also restored by Ft1. Conclusions The haemostatic effect of Notoginsenoside Ft1 was exerted through potentiation of PLC γ2‐ IP 3 / DAG ‐[Ca 2+ ]/ PKC ‐ TXA 2 signalling pathway stimulated by other stimulators. Notoginsenoside Ft1 has the potential to be developed into supplements in antiplatelet therapy to prevent the drawback of bleeding.