Open AccessPolysaccharide fraction isolated from P assiflora edulis inhibits the inflammatory response and the oxidative stress in mice
Author(s) -
Silva Renan O.,
Damasceno Samara R. B.,
Brito Tarcísio V.,
Dias Jordana M.,
Fontenele Amanda M.,
Braúna Isabela S.,
Júnior José S. C.,
Maciel Jeanny S.,
Paula Regina C. M.,
Ribeiro Ronaldo A.,
Souza Marcellus H. L. P.,
Freitas Ana L. P.,
Medeiros JandVenes R.,
Silva Draulio C.,
Barbosa André L. R.
Publication year - 2015
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1111/jphp.12399
Subject(s) - histamine , licking , myeloperoxidase , chemistry , oxidative stress , glutathione , vascular permeability , pharmacology , malondialdehyde , carrageenan , nociception , prostaglandin , prostaglandin e2 , biochemistry , inflammation , medicine , endocrinology , immunology , enzyme , receptor
Objectives The aim of the study was to investigate the anti‐inflammatory, antioxidant and antinociceptive actions of PFPe , a polysaccharide fraction isolated from the dried fruit of the P assiflora edulis . Methods Animals were pretreated with PFPe (0.3, 1 or 3 mg/kg, i.p.) 1 h before induction of paw oedema by carrageenan, histamine, serotonin, compound 48/80 or prostaglandin E 2 ( PGE 2). Neutrophil migration and vascular permeability were measured after carrageenan injection into the peritoneum, and the action of the PFPe on the tumour necrosis factor‐alpha, interleukin‐1 beta ( IL ‐1β), myeloperoxidase ( MPO ), glutathione ( GSH ) and malondialdehyde ( MDA ) levels was also evaluated. To assay nociception, we examined acetic acid‐induced writhing, formalin‐induced paw licking and response latency in the hot plate test. Key findings Pretreatment with PFPe significantly inhibited carrageenan‐induced paw oedema. PFPe also reduced paw oedema induced by compound 48/80, histamine, serotonin, and PGE 2 and compound 48/80‐induced vascular permeability. In addition, PFP e significantly reduced the MPO activity, MDA and GSH concentrations, and IL ‐1β level. In the nociception tests, PFPe reduced acetic acid‐induced writhing and formalin‐induced paw licking and did not increase the response latency time. Conclusions Our results suggest that PFPe administration reduces the inflammatory response by modulation of the liberation or synthesis of histamine and serotonin, by reduction of neutrophil migration, IL ‐1β levels, and oxidative stress and nociception.