Open AccessImmunoproteomic characterisation of M ycoplasma mycoides subspecies capri by mass spectrometry analysis of two‐dimensional electrophoresis spots and western blot
Author(s) -
Churchward Colin P.,
Rosales Rubén S.,
Gielbert Adriana,
Domínguez Mercedes,
Nicholas Robin A.J.,
Ayling Roger D.
Publication year - 2015
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1111/jphp.12344
Subject(s) - mycoplasma mycoides , western blot , purine nucleoside phosphorylase , biology , subspecies , microbiology and biotechnology , ribosomal protein , biochemistry , mycoplasma , ribosome , enzyme , purine , rna , paleontology , gene
Objectives M ycoplasma mycoides subspecies capri is one of the causative agents of contagious agalactia in goats. The disease is characterised by mastitis, pneumonia, arthritis, keratitis and in acute cases septicaemia. No vaccine is currently available that has been demonstrated to prevent disease. Methods This study used two‐dimensional electrophoresis to separate proteins from whole‐cell preparations and tandem mass spectrometry to identify them. Key findings In total, 145 spots were successfully identified corresponding to 74 protein identities. Twenty of these proteins were found to be immunogenic by western blot analysis using a pooled serum sample from experimentally infected goats. Conclusions Six proteins were found to have a less than 95% amino acid similarity to a closely related M ycoplasma species showing that they warrant further evaluation in development of diagnostic tests. These proteins were a dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex, phosphoglycerate kinase, pyrimidine‐nucleoside phosphorylase, 30 S ribosomal protein S 6, ribulose‐phosphate 3‐epimerase and D ‐lactate dehydrogenase.