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Challenges and advances in optical 3D mesoscale imaging
Author(s) -
Munck Sebastian,
Cawthorne Christopher,
EscamillaAyala Abril,
Kerstens Axelle,
Gabarre Sergio,
Wesencraft Katrina,
Battistella Eliana,
Craig Rebecca,
Reynaud Emmanuel G.,
Swoger Jim,
McConnell Gail
Publication year - 2022
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/jmi.13109
Subject(s) - mesoscale meteorology , opacity , microscopy , medical imaging , optical imaging , optics , computer science , physics , artificial intelligence , meteorology
Optical mesoscale imaging is a rapidly developing field that allows the visualisation of larger samples than is possible with standard light microscopy, and fills a gap between cell and organism resolution. It spans from advanced fluorescence imaging of micrometric cell clusters to centimetre‐size complete organisms. However, with larger volume specimens, new problems arise. Imaging deeper into tissues at high resolution poses challenges ranging from optical distortions to shadowing from opaque structures. This manuscript discusses the latest developments in mesoscale imaging and highlights limitations, namely labelling, clearing, absorption, scattering, and also sample handling. We then focus on approaches that seek to turn mesoscale imaging into a more quantitative technique, analogous to quantitative tomography in medical imaging, highlighting a future role for digital and physical phantoms as well as artificial intelligence.