Open AccessThe role of Gastric Mucosal Sulphydryls in the Ulcer‐protecting Effects of Cisapride
Author(s) -
López A.,
Motilva V.,
Lastra C. Alarcón,
Martín M. J.,
Casa C.
Publication year - 1996
Publication title -
journal of pharmacy and pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.745
H-Index - 118
eISSN - 2042-7158
pISSN - 0022-3573
DOI - 10.1111/j.2042-7158.1996.tb05873.x
Subject(s) - cisapride , benzamide , motility , ethanol , endogeny , chemistry , lesion , pharmacology , medicine , gastroenterology , biochemistry , biology , surgery , stereochemistry , genetics
The present study was designed to examine the role of endogenous sulphydryls (SHs) in the gastro‐protection induced by cisapride (CIS) (10, 25 and 50 mg kg −1 i.p.), a potent benzamide stimulating gastrointestinal motility in mucosal injury induced by 50% v/v ethanol. Results were compared with those of 5−hydroxytryptamine (5−HT) (10 mg kg −1 ). Ethanol mucosal damage was significantly reduced by treatment with CIS and 5−HT. On the contrary, administration of n ‐ethylmaleimide (NEM) (10 mg kg −1 ) an SH alkylator, markedly worsened lesion formation and counteracted the protective effect of CIS. Rats pretreated with CIS significantly increased the total sulphydryls as reflected in the non‐protein and protein fractions however, 5−HT treatment showed a fall in the non‐protein level. The present results suggest that 5−HT‐ergic dependent mechanisms have no relation to the gastro‐protection afforded by CIS in this experimental model. It is possible that mucosal SHs could be involved.