Open AccessHydrocortisone Diffusion Through Synthetic Membrane, Mouse Skin, and Epiderm™ Cultured Skin
Author(s) -
Christensen John Mark,
Chuong Monica Chang,
Le Hang,
Pham Loan,
Bendas Ehab
Publication year - 2011
Publication title -
archives of drug information
Language(s) - English
Resource type - Journals
ISSN - 1753-5174
DOI - 10.1111/j.1753-5174.2010.00033.x
Subject(s) - penetration (warfare) , permeation , membrane , human skin , chemistry , chromatography , biophysics , dermatology , medicine , biology , biochemistry , operations research , engineering , genetics
Objectives. The penetration of hydrocortisone (HC) from six topical over‐the‐counter products along with one prescription cream through cultured normal human‐derived epidermal keratinocytes (Epiderm™), mouse skin and synthetic nylon membrane was performed as well as the effect hydrating the skin by pre‐washing was explored using the Upright Franz Cell. Method and Results. Permeation of HC through EpiDerm™, mouse skin and synthetic membrane was highest with the topical HC gel formulation with prewash treatment of the membranes among seven products evaluated, 198 ± 32 µg/cm 2 , 746.32 ± 12.43 µg/cm 2 , and 1882 ± 395.18 µg/cm 2 , respectively. Pre‐washing to hydrate the skin enhanced HC penetration through EpiDerm™ and mouse skin. The 24‐hour HC released from topical gel with prewash treatment was 198.495 ± 32 µg/cm 2 and 746.32 ± 12.43 µg/cm 2 while without prewash, the 24‐h HC released from topical gel was 67.2 ± 7.41 µg/cm 2 and 653.43 ± 85.62 µg/cm 2 though EpiDerm™ and mouse skin, respectively. HC penetration through synthetic membrane was ten times greater than through mouse skin and EpiDerm™. Generally, the shape, pattern, and rank order of HC diffusion from each commercial product was similar through each membrane.