Open AccessThe Mechanism of Uptake of Ascorbic Acid into Osteoblasts and Leukocytes
Author(s) -
Wolf George
Publication year - 1996
Publication title -
nutrition reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.958
H-Index - 150
eISSN - 1753-4887
pISSN - 0029-6643
DOI - 10.1111/j.1753-4887.1996.tb03918.x
Subject(s) - ascorbic acid , dehydroascorbic acid , chemistry , biochemistry , intracellular , hydroxyproline , transporter , osteoblast , in vitro , gene , food science
Ascorbic acid is taken up into osteoblast cells by a saturable, stereospecific, Na + ‐dependent transporter, accumulating ascorbic acid to a level 100‐fold that in the medium. The ascorbic acid uptake rate correlated with intracellular hydroxyproline synthesis. A second, distinct mechanism has also been described for accumulation of ascorbic acid into neutrophils and myeloid leukemia cells. This appears to be Na + ‐independent and relies on the glucose transporter GLUT1 to ferry dehydroascorbic acid (DHA) into cells and then to trap it as ascorbic acid to a high concentration.