Open AccessEffects of Irradiation on Cementum Matrix Cytokins Function during Periodontal Regeneration
Author(s) -
NISHIMURA Kazuaki,
TSUCIYA Hirofumi,
TAKADA Kohei,
KISHIMOTO Takuma,
IMAI Hisao,
MIKAMI Yoshimasa,
MUNEKATA Masanobu
Publication year - 2003
Publication title -
human cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.893
H-Index - 37
eISSN - 1749-0774
pISSN - 0914-7470
DOI - 10.1111/j.1749-0774.2003.tb00156.x
Subject(s) - cementum , periodontal fiber , cementogenesis , regeneration (biology) , chemistry , microbiology and biotechnology , dental alveolus , alkaline phosphatase , dentistry , biology , biochemistry , medicine , dentin , enzyme
The influence of γ‐ray irradiation on a cementum‐impregnated gelatine membrane (CGM) was analyzed with emphasis on its function during periodontal regeneration. In brief, proteins were extracted from γ‐ray irradiated cementum (γ C). With the γ C protein, sample cells (gingival fibroblasts, periodontal ligament cells, and alveolar bone cells) were co‐cultured, and cytological parameters (cell attachment, cell differentiation and alkaline phosphatase activity) were analyzed. Additionally, kinetics of some gene expression was analysed using reverse transcript RT‐PCR, which included osteoproteogerin (OPG) / osteoclastogenesis inhibitory factor (OCIF) mRNA. BMP‐2 and osteonectin were resistant to γ ‐rays, and other cytokines involved in regeneration were decreased. Thus, the attachment activity of osteoblasts to γ C protein was higher than that of non‐irradiated cementum (control C). The expression of OPG/OCIF mRNA was lower in co‐cultured cells with γ C protein than those with in control C protein. Together the results imply that some cytokine in intact cementum prevents the attachment (differentiation) of bone cells onto the root surface, which may explain why the introduction of CGM following gingival flap surgery induces new cementum, new ligament and new bone formation, but CGM irradiated with γ ‐rays for clinical use causes ankylosis.