Open AccessImproved method to raise polyclonal antibody using enhanced green fluorescent protein transgenic mice
Author(s) -
Ren Jianke,
Wang Long,
Liu Guoxiang,
Zhang Wen,
Sheng Zhejin,
Wang Zhugang,
Fei Jian
Publication year - 2008
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2008.00381.x
Subject(s) - polyclonal antibodies , antiserum , green fluorescent protein , fusion protein , antibody , titer , transgene , epitope , genetically modified mouse , microbiology and biotechnology , recombinant dna , biology , antigen , antibody titer , virology , chemistry , immunology , biochemistry , gene
Recombinant fusion protein is widely used as an antigen to raise antibodies against the epitope of a target protein. However, the concomitant anticarrier antibody in resulting antiserum reduces the production of the desired antibody and brings about unwanted non‐specific immune reactions. It is proposed that the carrier protein transgenic animal could be used to solve this problem. To validate this hypothesis, enhanced green fluorescent protein (EGFP) transgenic mice were produced. By immunizing the mice with fusion protein His6HAtag‐EGFP, we showed that the antiserum from the transgenic mice had higher titer antibody against His6HA tag and lower titer antibody against EGFP compared with that from wild‐type mice. Therefore, this report describes an improved method to raise high titer antipeptide polyclonal antibody using EGFP transgenic mice that could have application potential in antibody preparation.