Open AccessEffect of Human WEE1 and Stem Cell Factor on Human CD34 + Umbilical Cord Blood Cell Damage Induced by Chemotherapeutic Agents
Author(s) -
LEI Ping,
HE Yong,
SHI Wenfang,
PENG Jilin,
WU Sha,
ZHU Huifen,
CHEN Jianguo,
SHEN Guanxin
Publication year - 2007
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2007.00312.x
Subject(s) - stem cell factor , stem cell , cisplatin , pharmacology , doxorubicin , cd34 , apoptosis , viability assay , chemistry , biology , cancer research , microbiology and biotechnology , haematopoiesis , chemotherapy , biochemistry , genetics
Myelosuppression is one of the major side‐effects of most anticancer drugs. To achieve myeloprotection, one bicistronic vector encoding anti‐apoptotic protein human WEE1 (WEE1Hu) and proliferation‐stimulating stem cell factor (SCF) was generated. In this study, we selected human umbilical cord blood CD34 + cells as the in vitro model in an attempt to investigate whether WEE1Hu , rather than conventional drug‐resistant genes, can be introduced to rescue cells from the damage by chemotherapeutic agents such as cisplatin, adriamycin, mitomycin‐c and 5‐fluorouracil. Cell viability and cytotoxicity assay, colony‐forming units in culture assay and externalization of phospholipid phosphatidylserine analysis showed that the expression of WEE1Hu and SCF in CD34 + cells provided the cells with some protection. These findings suggest that the expression of WEE1Hu and SCF might rescue CD34 + cells from chemotherapy‐induced myelosuppression.