Open AccessA Lectin from Chinese Mistletoe Increases γδ T Cell‐mediated Cytotoxicity through Induction of Caspase‐dependent Apoptosis
Author(s) -
GONG Fang,
MA Yanhui,
MA Anlun,
YU Qiwen,
ZHANG Jiying,
NIE Hong,
CHEN Xuehua,
SHEN Baihua,
LI Ningli,
ZHANG Dongqing
Publication year - 2007
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2007.00300.x
Subject(s) - cytotoxicity , apoptosis , phosphatidylserine , microbiology and biotechnology , cytotoxic t cell , jurkat cells , dna fragmentation , t cell , cytokine , biology , tumor necrosis factor alpha , chemistry , programmed cell death , immunology , biochemistry , immune system , phospholipid , in vitro , membrane
In this study, a mistletoe lectin (ML) was purified from Chinese mistletoe and the effect of this 60 kDa Chinese ML on human γδ T cell cytotoxicity, apoptosis and modulation of the cytokine network was studied. The cytotoxic properties of δ T cells was evaluated by using a 51 Cr release test and employed fluorescence‐activated cell sorting and enzyme‐linked immunosorbent assay analysis to quantify translocation of the cell membrane phospholipid, phosphatidylserine and nuclear DNA fragmentation during apoptosis. It was found that: (i) ML effectively stimulated γδ T cell proliferation in a dose‐ and time‐dependent manner; (ii) ML increased γδ T cell cytotoxicity; (iii) ML could modulate lipopolysaccharide‐induced cytokine release in a pro‐inflammatory manner by increasing tumor necrosis factor (TNF)‐α release and inhibiting the release of anti‐inflammatory interleukin (IL)‐10: (iv) ML induced apoptosis in caspase‐dependent and CD95‐independent manner. The results indicated that ML is a potent immunomodulator to human γδ T cell cytotoxicity, apoptosis and cytokine production.