Open AccesshELP3 Subunit of the Elongator Complex Regulates the Transcription of HSP70 Gene in Human Cells
Author(s) -
HAN Qiuju,
HOU Xiaozhe,
SU Dongmei,
PAN Lina,
DUAN Jizhou,
CUI Liguo,
HUANG Baiqu,
LU Jun
Publication year - 2007
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2007.00293.x
Subject(s) - gene expression , biology , microbiology and biotechnology , gene , regulation of gene expression , protein subunit , transcription (linguistics) , rna polymerase ii , hsp70 , transcriptional regulation , genetics , heat shock protein , promoter , linguistics , philosophy
The human Elongator complex is remarkably similar to its yeast counterpart in several aspects. In a previous study, we analyzed the functions of the human elongation protein 3 (hELP3) subunit of the human Elongator by using an in vivo yeast complementation system. However, direct evidence for hELP3 functions in regulating gene expression in human cells was not obtained. In this study, we used hELP3 antisense oligonucleotide inhititors to knock down hELP3 gene expression to investigate its function in human 293T cells. The results showed that specific reduction of hELP3 mRNA and protein caused a significant suppression of HSP70‐2 gene expression, and this was accompanied by histone H3 hypoacetylation and decreased RNA polymerase II density at the HSP70‐2 gene. Moreover, the data also showed that hELP3 exerted the transcriptional regulatory function directly through its presence on the HSP70‐2 gene. Data presented in this report provide further insight and direct evidence of the functions of hELP3 in HSP70‐2 gene transcriptional elongation in human cells.