Open AccessSynergy between Cucumber Mosaic Virus and Zucchini Yellow Mosaic Virus on Cucurbitaceae Hosts Tested by Real‐time Reverse Transcription‐Polymerase Chain Reaction
Author(s) -
ZENG Rong,
LIAO Qiansheng,
FENG Junli,
LI Dingjun,
CHEN Jishuang
Publication year - 2007
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2007.00292.x
Subject(s) - orfs , zucchini yellow mosaic virus , cucumber mosaic virus , biology , virology , virus , plant virus , open reading frame , gene , genetics , peptide sequence
Cucumber mosaic virus (CMV) and zucchini yellow mosaic virus (ZYMV) are two principal viruses infecting cucurbitaceous crops, and their synergy has been repeatedly observed. In our present work, a real‐time reverse transcription‐polymerase chain reaction procedure was established to study the accumulation kinetics of these two viruses in single and combined infections at the molecular level. The accumulations of open reading frames (ORFs) for 1a, 2a, 3a and coat protein (CP) of CMV and CP of ZYMV were tested. In the single infection, CMV‐Fny ORFs accumulated to their maxima in cucumber or bottle gourd at 14 d post‐inoculation (dpi), and gradually declined thereafter. ZYMV‐SD CP ORF reached maximal accumulation at 14 d post‐inoculation (dpi), and gradually declined thereafter. ZYMV‐SD CP ORF reached maximal accumulation at 14 and 28 dpi on cucumber and bottle gourd, respectively. However, when coinfected with CMV‐Fny and ZYMV‐SD, the maximal accumulation levels of all viral ORFs were delayed. CMV‐Fny ORFs reached their maxima at 21 dpi on both hosts, and ZYMV‐SD CP ORF reached maximal accumulation at 21 and 28 dpi on cucumber and bottle gourd, respectively. Generally, the accumulation levels of CMV‐Fny ORFs in the co‐infection were higher than those in the single infection, whereas the accumulation of ZYMV‐SD CP ORF showed a reverse result.