Open AccessNovel Tumor‐associated Antigen of Hepatocellular Carcinoma Defined by Monoclonal Antibody E4‐65
Author(s) -
ZOU Ke,
JU Jihang,
XIE Hong
Publication year - 2007
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2007.00288.x
Subject(s) - monoclonal antibody , antigen , hepatocellular carcinoma , antibody , immunohistochemistry , immunofluorescence , cell culture , blot , microbiology and biotechnology , biology , western blot , cell , pathology , cancer research , immunology , medicine , biochemistry , gene , genetics
A monoclonal antibody, E4‐65, produced by immunizing mice with SMMC‐7721 cells, a human hepatocellular carcinoma (HCC) cell line, was used to identify and characterize an unreported HCC‐associated antigen. Indirect immunofluorescence studies showed that E4‐65 antibody reacted with five out of eight HCC cell lines, but not with 10 non‐HCC tumor cell lines or a normal liver cell line. Using immunohistochemical examination, E4‐65 antigen was detected on the cell membranes and in the cytoplasm of human liver tumor tissues, but was not found in most other tumors, or normal adult or fetal tissues, except for a weakly positive reaction in tissues of the digestive system. Western blot analysis showed that E4‐65 antibody bound to a 45 kDa protein in the human HCC cell line and tissue lysates. Enzyme treatment and lectin blotting did not detect the carbohydrate chain in E4‐65 antigen. This HCC‐associated protein represents a potentially useful target for diagnoses and immunotherapy of human HCC.