Open AccessCloning and Characterization of Genes Encoded in dTDP‐ D ‐mycaminose Biosynthetic Pathway from a Midecamycin‐producing Strain, Streptomyces mycarofaciens
Author(s) -
CONG Lina,
PIEPERSBERG Wolfgang
Publication year - 2007
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2007.00265.x
Subject(s) - gene , streptomyces fradiae , biology , biosynthesis , polyketide synthase , cloning (programming) , genetics , biochemistry , microbiology and biotechnology , streptomyces , polyketide , actinomycetales , bacteria , computer science , programming language
Two subclusters from Streptomyces mycarofaciens , a midecamycin producer, were cloned and partially sequenced. One region was located at the 5′ end of the mid polyketide synthase (PKS) genes and contained the genes midA , midB and midC. The other region was at the 3′ end of the PKS genes and contained midK , midI and midH. Analysis of the nucleotide sequence revealed that these genes encode dTDP‐glucose synthase ( midA ), dTDP‐glucose dehydratase ( midB ), aminotransferase ( midC ), methyltransferase ( midK ), glycosyltransferase ( midI ) and an assistant gene ( midH ). All of these genes are involved in the biosynthesis of dTDP‐ D ‐mycaminose, the first deoxysugar of midecamycin, and in transferring the mycaminose to the midecamycin aglycone in S. mycarofaciens. Similar to gene pairs desVIII/desVII in S. venezuelae and tylMIII/tylMII in S. fradiae , the product of midH probably functions as an auxiliary protein required by the MidI protein for efficient glycosyltransfer in midecamycin biosynthesis.