Open AccessApplication of Cell Penetrating Peptide in Magnetic Resonance Imaging of Bone Marrow Mesenchymal Stem Cells
Author(s) -
LIU Min,
GUO YouMin,
YANG JunLe,
WANG Peng,
ZHAO LinYu,
SHEN Nian,
WANG SiCen,
GUO XiaoJuan,
WU QiFei
Publication year - 2006
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2006.00239.x
Subject(s) - mesenchymal stem cell , magnetic resonance imaging , fluorescein isothiocyanate , stem cell , chemistry , bone marrow , cell , intracellular , live cell imaging , peptide , microbiology and biotechnology , in vitro , biophysics , pathology , biology , biochemistry , medicine , fluorescence , physics , quantum mechanics , radiology
Tracking the distribution and differentiation of stem cells by high‐resolution imaging techniques would have significant clinical and research implications. In this study, a model cell‐penetrating peptide was used to carry gadolinium particles for magnetic resonance imaging (MRI) of mesenchymal stem cells (MSCs). MSCs were isolated from rat bone marrow and identified by osteogenic differentiation in vitro . The cell‐penetrating peptide labeled with fluorescein‐5‐isothiocyanate (FITC) and gadolinium was synthesized by a solid‐phase peptide synthesis method. Fluorescein imaging analysis confirmed that this new peptide could internalize into the cytoplasm and nucleus at room temperature, 4°C and 37°C. Gadolinium were efficiently internalized into mesenchymal stem cells by the peptide in a time or concentration‐dependent manner, resulting in intercellular shortening of longitudinal relaxation enhancements, which were obviously detected by 1.5 Tesla Magnetic Resonance Imaging. Cytotoxicity assay and flow cytometric analysis showed that the intercellular contrast medium incorporation did not affect cell viability at the tested concentrations. The in vitro experiment results suggested that the new constructed peptides could be a vector for tracking MSCs. Edited by Ming‐Hua XU