Open AccessTransient Decrease of Light‐harvesting Complex II Phosphorylation Level by Hypoosmotic Shock in Dark‐adapted Dunaliella salina
Author(s) -
LIU XianDe,
HU FenHong,
SHEN YunGang
Publication year - 2006
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2006.00139.x
Subject(s) - dunaliella salina , phosphorylation , dephosphorylation , dunaliella , biology , intracellular , protein phosphorylation , darkness , biochemistry , biophysics , phosphatase , microbiology and biotechnology , algae , protein kinase a , botany
This study investigated the regulation of major light harvesting chlorophyll a/b protein (LHCII) phosphorylation by hypoosmotic shock in dark‐adapted Dunaliella salina cells. When the external NaCl concentration decreased in darkness, D. salina LHCII phosphorylation levels transiently dropped within 20 min and then restored gradually to basal levels. The transient decrease in LHCII phosphorylation levels was insensitive to NaF, a phosphatase inhibitor. Inhibition of intracellular ATP production by addition of an uncoupler or an ATP synthase inhibitor increased LHCII phosphorylation levels in D. salina cells exposed to hypoosmotic shock. Taken together, these results indicate that hypoosmotic shock inhibits the LHCII phosphorylation process. The related mechanism and physiological significance are discussed. Edited by
Ming‐Hua XU