Open AccessProfiling of Differentially Expressed Genes in LRRC4 Overexpressed Glioblastoma Cells by cDNA Array
Author(s) -
ZHANG QiuHong,
WU MingHua,
WANG LiLi,
CAO Li,
TANG Ke,
PENG Cong,
GAN Kai,
LI XiaoLing,
LI GuiYuan
Publication year - 2005
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2005.00100.x
Subject(s) - gene knockdown , biology , cell growth , carcinogenesis , transfection , gene , complementary dna , cell culture , microbiology and biotechnology , cancer research , genetics
Our previous study has shown that LRRC4 is a novel member of the leucine‐rich repeat (LRR) superfamily and has the potential to suppress brain tumor growth. In order to further analyze the functions of LRRC4 on the maintenance of normal function and suppression of tumorigenesis in the central nervous system, we investigated alterations in gene expression related to neurobiology by the Atlas array in two inducible dual‐stable LRRC4 ‐overexpressing cell lines. Seventeen of 588 genes spotted on the Atlas membrane showed altered expression levels in LRRC4 transfected U251MG Tet‐on cells, which are involved in cell proliferation and cell cycle progression, tumor invasion and metastasis, and neurotransmitter synthesis and release. In addition, cell invasion assay results showed that LRRC4 can inhibit the U251MG cell migration. These studies represent the first cDNA array analysis of the effects of LRRC4 on the involvement of different neurobiological genes in U251MG glioblastoma cells and provide new insights into the function of LRRC4 in glioma. Edited by
Yao LI