Open AccessStudy of a Novel Brain Relatively Specific Gene LRRC4 Involved in Glioma Tumorigenesis Suppression Using the Tet‐on System
Author(s) -
ZHANG QiuHong,
WANG LiLi,
CAO Li,
PENG Cong,
LI XiaoLing,
TANG Ke,
LI WeiFang,
LIAO Ping,
WANG JieRu,
LI GuiYuan
Publication year - 2005
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2005.00079.x
Subject(s) - cell growth , biology , cell cycle , carcinogenesis , cancer research , glioma , cyclin d1 , retinoblastoma , microbiology and biotechnology , chemistry , cell , gene , genetics
Abstract LRRC4 is a novel relatively specific gene, which displays significant down‐regulation in primary brain tumor biopsies and has the potential to suppress brain tumor growth. In this study, we investigated the growth inhibitory effect of LRRC4 on tumorigencity in vivo and on cell proliferation in vitro by a tetracycline‐inducible expression system. Results showed that LRRC4 significantly reduced the growth and malignant grade of xenografts arising from glioblastoma U251MG cells. Cell proliferation was markedly inhibited after U251MG Tet‐on‐LRRC4 cell induction with doxycycline. Flow cytometry and Western blot analysis demonstrated that LRRC4 mediated a delay of the cell cycle in late G1, possibly through up‐regulating the expressions of p21Waf1/cip1 and p27Kip1 and down‐regulating the expressions of cyclin‐dependent kinase 2, retinoblastoma protein and epidermal growth factor receptors. Together, these findings provide clues to the function of LRRC4 as a negative regulator of cell growth and underscore a link between the above‐mentioned cyclins, cyclin‐associated molecules and tumorigenicity. Edited by
Da‐Fang WAN