Open AccessBAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase II in Gene Transcriptional Activation
Author(s) -
ZHAO LiHui,
BA XueQing,
WANG XiaoGuang,
ZHU XiaoJuan,
WANG Li,
ZENG XianLu
Publication year - 2005
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2005.00061.x
Subject(s) - rna polymerase ii , chromatin immunoprecipitation , microbiology and biotechnology , transcription (linguistics) , hela , gene , rna polymerase , chromatin , biology , transcription factor , rna , chemistry , gene expression , transcriptional regulation , nucleosome , promoter , genetics , in vitro , linguistics , philosophy
Brg‐or hBrm‐associated factor (BAF) complexes, a chromatin‐remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg‐associated factor complexes. In the present study, we investigated the spatial relationship between Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase II (RNAP II) upon gene transcriptional activation in vivo by employing immuno‐gold labeling. The data showed that Brg1 was closely co‐localized with NF1/CTF and RNAP II in HeLa cells. Moreover, the co‐immunoprecipitation assay further revealed that Brg1 can be isolated together with NF1/CTF and RNAP II in the ConA‐stimulated, but not the resting, T lymphocyte. The combined results suggested that BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription. Edited by You‐Shang ZHANG