Open AccessAnaerobic Induction of Isocitrate Lyase and Malate Synthase in Submerged Rice Seedlings Indicates the Important Metabolic Role of the Glyoxylate Cycle
Author(s) -
LU Ying,
WU YongRui,
HAN Bin
Publication year - 2005
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2005.00060.x
Subject(s) - glyoxylate cycle , malate synthase , isocitrate lyase , citric acid cycle , biochemistry , malate dehydrogenase , citrate synthase , metabolism , biology , tricarboxylic acid , enzyme , lyase , isocitrate dehydrogenase , chemistry
The glyoxylate cycle is a modified form of the tricarboxylic acid cycle that converts C2 compounds into C4 dicarboxylic acids at plant developmental stages. By studying submerged rice seedlings, we revealed the activation of the glyoxylate cycle by identifying the increased transcripts of mRNAs of the genes of isocitrate lyase (ICL) and malate synthase (MS), two characteristic enzymes of the glyoxylate cycle. Northern blot analysis showed that ICL and MS were activated in the prolonged anaerobic environment. The activity assay of pyruvate decarboxylase and ICL in the submerged seedlings indicated an 8.8‐fold and 3.5‐fold increase over that in the unsubmerged seedlings, respectively. The activity assay of acetyl‐coenzyme A synthetase in the submerged seedlings indicated a 3‐fold increase over that in the unsubmerged seedlings, which is important for initiating acetate metabolism. Consequently, we concluded that the glyoxylate cycle was involved in acetate metabolism under anaerobic conditions. Edited by: Hong‐Xuan LIN