Open AccessCarboxyfluorescein Diacetate Succinimidyl Ester Fluorescent Dye for Cell Labeling
Author(s) -
WANG XiaoQi,
DUAN XiuMei,
LIU LiHua,
FANG YanQiu,
TAN Yan
Publication year - 2005
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2005.00051.x
Subject(s) - fluorescence , chemistry , viability assay , cell , staining , biophysics , fluorescein , biochemistry , biology , physics , genetics , quantum mechanics
Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA‐SE) and the methodology of cell labeling using CFDA‐SE fluorescent dye. First, we analyzed the kinetics of CFDA‐SE fluorescent dye intensity over time. Second, we determined the optimal concentration of CFDA‐SE fluorescent dye for cell labeling. Third, we tested the toxicity of CFDA‐SE fluorescent dye on labeled cells. Finally, we determined the optimal staining time of CFDA‐SE fluorescent dye for cell labeling. The results show that the optimal concentration of CFDA‐SE fluorescent dye for cell labeling varies according to different cell types. CFDA‐SE fluorescent dye is non‐toxic to cells as the cell death rate caused by CFDASE labeling is below 5%. The optimal cell labeling time was determined to be 8 min of incubation with CFDA‐SE fluorescent dye. We concluded that the advantages of using CFDA‐SE fluorescent dye for cell labeling are as follows: (1) the binding of CFDA‐SE fluorescent dye to cells is stable; (2) CFDA‐SE fluorescent dye is not toxic and does not modify the viability of labeled cells; and (3) CFDA‐SE fluorescent dye is a suitable fluorochrome for cell labeling. Edited by: Xue‐Liang ZHU