Open AccessApplication of Phage‐displayed Single Chain Antibodies in Western Blot
Author(s) -
DING YanLi,
LIU MeiYun,
HAN Wei,
YANG ShengLi,
LIU Hui,
GONG Yi
Publication year - 2005
Publication title -
acta biochimica et biophysica sinica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.771
H-Index - 57
eISSN - 1745-7270
pISSN - 1672-9145
DOI - 10.1111/j.1745-7270.2005.00018.x
Subject(s) - phage display , panning (audio) , antibody , microbiology and biotechnology , antigen , biopanning , western blot , virology , biology , titer , dot blot , single chain , peptide library , dna , peptide sequence , gene , genetics , paleontology , zoom , lens (geology)
A phage display single chain fragment variable library constructed on pIII protein of M13 filamentous phage was screened using B‐lymphocyte stimulator and FP248 as selective molecules. After four rounds of panning, there was a remarkable enrichment in the titer of bound phages. Twenty phage clones were selected from the last round and screened by means of phage‐ELISA. With the antibody phages as primary antibodies in Western blot, we developed a method for detecting the specific antigen. The dilutions of antibody phages depend on the affinity between antibody‐displayed phage particles and antigens. Edited by Ding‐Gan LIU