Open AccessSpatial and temporal expression of c‐mos in mouse testis during postnatal development
Author(s) -
Cao ShaoFeng,
Li Ding,
Yuan Qing,
Guan Xin,
Xu Chen
Publication year - 2008
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2008.00324.x
Subject(s) - in situ hybridization , messenger rna , biology , blot , microbiology and biotechnology , spermatogenesis , gene expression , cytoplasm , polyclonal antibodies , nucleus , andrology , gene , endocrinology , antibody , genetics , medicine
Aim: To immunolocalize the c‐mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development. Methods: Semi‐quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) and in situ hybridization techniques were used to examine c‐mos mRNA and indirect immunofluorescence was used to localize c‐Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70. Results: c‐mos mRNA remained low on postnatal days 14–21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c‐mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti‐c‐Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate. c‐Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c‐Mos‐positive spermatids increased progressively till day 49 and stabilized thereafter. Conclusion: The c‐mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c‐mos might play important roles in spermatogenesis.