Open AccessInhibition of mouse acrosome reaction and sperm‐zona pellucida binding by anti‐human sperm membrane protein 1 antibody
Author(s) -
Cheng GuoYan,
Shi JianLi,
Wang Min,
Hu YanQin,
Liu ChunMeng,
Wang YiFei,
Xu Chen
Publication year - 2007
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2007.00247.x
Subject(s) - zona pellucida , sperm , acrosome reaction , human fertilization , acrosome , antibody , biology , microbiology and biotechnology , andrology , recombinant dna , oocyte , chemistry , immunology , gene , biochemistry , genetics , embryo , medicine
Aim : To investigate the possible functions of human sperm membrane protein (hSMP‐1) in the process of fertilization. Methods : A 576‐bp cDNA fragment of HSD‐1 gene coding for the extracellular domain of hSMP‐1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti‐recombinant hSMP‐1 (anti‐rhSMP‐1) antibodies. Sperm acrosome reaction and sperm‐zona pellucida (ZP) binding assay were carried out in 10‐week‐old BALB/c mice. Results : Recombinant hSMP‐1 was successfully cloned and expressed. The expression of the native protein was limited on the acrosome of human and mouse sperm. Treatment of anti‐rhSMP‐1 antibodies significantly decreased the average number of sperms bound to each egg. Meanwhile, the percentage of acrosome reaction was decreased in comparison to pre‐immune control after treatment with anti‐rhSMP‐1 ( P < 0.05). Conclusion : The results suggest that anti‐rhSMP‐1 antibody inhibited mouse acrosome reaction and sperm‐ZP binding. Edited by De‐Yi Liu